Mastitis is the most economically significant disease of dairy animals

s: In the present study, 20 samples of milk of cow, pasteurized milk were collected from the local market and were analyzed for microbial count and IMViC tests to determine the coliform load in the sample. Further, the presence of E. coli (KC795687) was confirmed by using PCR and 16srna sequencing. Majority of the milk samples of different origin were found to be contaminated by the coliform group of bacteria. The samples were found to be positive for E. coli by PCR analysis and 16srna sequencing. Pasteurized milk samples did not showed presence of E. coliby PCR, but they showed considerable count of bacterial growth by total plate count method. The results indicated that analyzed milk could contribute a potential risk for public health in the cases that it was consumed or used in the production of dairy products without being pasteurized or being subjected to a sufficient heat process. Moreover, PCR is less labor intensive, more rapid for bacterial identification and it further confirmed in 16srna sequencing. Key Words; PCR-Polymeric chain Reaction, IMViC-Indole methyl red, vogues proskaur, citrate utilization test.